Key Steps in Protein Expression and Purification

✅ Gene Cloning and Vector Preparation – Clone the gene of interest into an appropriate expression vector that suits the host system (e.g., bacterial, yeast, mammalian cells).

✅ Transformation/Transfection – Introduce the recombinant vector into a suitable host organism (e.g., E. coli, yeast, mammalian cells) through transformation or transfection methods.

✅ Expression Induction – Induce protein expression in the host cells using inducers (e.g., IPTG for bacterial expression) to trigger the production of the target protein.

✅ Cell Harvesting – Collect the host cells after a specific incubation period to ensure optimal protein expression.

✅ Cell Lysis – Lyse the harvested cells to release the intracellular protein. Methods like sonication, freeze-thaw cycles, or enzyme-based lysis can be used.

✅ Protein fraction Extraction – After cell lysis, centrifuge the lysate to separate the soluble fraction (which contains the target protein) from the insoluble fraction (which contains cell debris and aggregates). The soluble fraction is typically used for further purification.

✅ Affinity Chromatography – Use a specific affinity tag (e.g., His-tag, GST-tag) on the protein to purify it using affinity chromatography. This technique selectively binds the target protein to a column, allowing contaminants to be washed away.

✅ Ion Exchange Chromatography – Further purify the protein by using ion exchange chromatography, which separates proteins based on their charge.

✅ Size Exclusion Chromatography – Purify the protein by separating it based on its size, typically used to achieve further homogeneity.

✅ Concentration and Buffer Exchange – Concentrate the purified protein and exchange it into an appropriate buffer for downstream applications (e.g., storage, functional assays).

✅ Verification of Purity and Activity – Analyze the purified protein’s purity using SDS-PAGE or Western blot, and confirm its functionality using activity assays or structural studies.

“From Gene to Function – High-Quality Protein Expression & Purification.”

Protein Expression and purification Services

Unlock the Potential of Your Research with Our Protein Expression and Purification Services

We specialize in providing high-quality protein expression and purification services tailored to your research and industrial needs. Whether you are studying protein function, structure, or preparing for downstream applications, our comprehensive services ensure that your proteins are produced efficiently, with purity and consistency.

Our Protein Expression & Purification Services Include:

  • Custom Protein Expression
    We offer protein expression in a wide range of systems, including bacterial (E. coli), yeast, and insect cells to accommodate your specific protein requirements.
  • Recombinant Protein Production
    We generate recombinant proteins using various expression vectors to ensure optimal yield and functionality, whether for structural studies, functional assays, or therapeutic applications.
  • Protein Purification (Tag-Free or Tagged Proteins)
    Our protein purification services utilize advanced chromatography techniques such as affinity chromatography, size exclusion chromatography, ion exchange, and more, to isolate high-purity proteins. We offer both tag-free and tagged protein purification options depending on your research needs.
  • Protein Refolding & Solubility Optimization
    For inclusion body-derived proteins, we provide refolding services to recover biologically active proteins and enhance solubility, ensuring proper folding and functionality.
  • Protein Characterization
    After purification, we offer detailed protein analysis and characterization services, including SDS-PAGE, Western blotting, mass spectrometry, and enzymatic activity assays, to confirm purity and functionality.

Why Choose Us?

  • Tailored Solutions:
    We understand that every protein is unique. Our services are customized to fit your specific needs, with flexible solutions for a wide range of protein types and research goals.
  • High Purity & Yield:
    Our purification processes are designed to maximize both protein purity and yield, ensuring you receive high-quality material for your experiments or applications.
  • Fast Turnaround:
    We know that time is crucial in research and development. Our efficient service ensures fast turnaround times without compromising quality, enabling you to move forward with your project on schedule.

Our Process

  1. Project Consultation:
    We begin by discussing your protein expression and purification needs to determine the best approach for your project. Whether it’s a challenging protein or a standard project, we will guide you through the optimal system and strategy.
  2. Gene Cloning & Expression System Setup:
    Based on your gene of interest, we select the most suitable expression system and vector. We then proceed with cloning and transformation to create the expression construct.
  3. Protein Expression:
    Once the expression system is set up, we induce protein production under optimal conditions to maximize yield while maintaining protein activity.
  4. Protein Purification:
    After successful expression, we employ a range of purification techniques to isolate your target protein, ensuring the highest level of purity and functionality.
  5. Characterization & Quality Control:
    Purified proteins are thoroughly analyzed for purity and activity using SDS-PAGE, mass spectrometry, and other techniques. We ensure that your protein meets the specifications required for your research.
  6. Delivery of Purified Protein:
    Once purified and characterized, the protein is delivered in the format required (e.g., liquid or lyophilized) for your downstream applications.

Get Started Today

Whether you are in academia, biotechnology, or pharmaceuticals, our protein expression and purification service can help accelerate your research and product development.

Please enable JavaScript in your browser to complete this form.
Name
X